isolation and culture of mesenchymal stem cells from rabbit scapular subcutaneous adipose tissue and their ability to differentiate into osteoblasts

Authors

hassan semyari periodontics department, dental school, shahed university, tehran, ir iranسازمان اصلی تایید شده: دانشگاه شاهد (shahed university)

mahmood rajipour periodontics department, dental school, shahed university, tehran, ir iran; periodontics department, dental school, shahed university, tehran, ir iran. tel: +98-2188959210, fax: +98-2188954915سازمان اصلی تایید شده: دانشگاه شاهد (shahed university)

farshid bastami dental school, shahed unieversity, tehran, ir iranسازمان اصلی تایید شده: دانشگاه شاهد (shahed university)

hossein semyari dental school, shahed unieversity, tehran, ir iranسازمان اصلی تایید شده: دانشگاه شاهد (shahed university)

abstract

conclusions based on our findings, we conclude that the separation and reproduction of adipose tissue cells is an appropriate method for purification of mscs in animal studies. regarding the histomorphometric and flow cytometry analysis results, we demonstrated the differentiation ability of mscs in normal medium and hope to employ these cells for the regeneration of damaged bone tissues in the future. results the identity of adipose tissue cells was confirmed by oil-red o staining and examination under an optical microscope at both the initial stage and after differentiation into mesenchymal cells. the results demonstrated that cells derived from adipose tissue differentiated into mesenchymal cells. the nature of the mesenchymal cells was confirmed by the expression of specific surface markers, including cd90, cd45, cd44, cd73, and cd105, by flow cytometry. finally, alizarin red staining confirmed the differentiation of mscs into osteoblasts. materials and methods sterile adipose tissue was obtained from the scapular subcutaneous adipose tissue of two rabbits (average weight, 2.8 kg) for cultivation and differentiation by either liposuction with a blunt hallow tip cannula or by direct surgery. the morphology, differentiation, and expression of mesenchymal-specific surface markers of rabbit, such as cd90, cd45, cd73, cd44, and cd105, were examined in cells from the third passage by flow cytometry. the mscs from adipose tissue were stained with a lentivirus genome for cell tracking. the differentiation of mscs into osteoblasts was investigated using a specific histological stain, alizarin red. background the objectives of this study were to separate and culture mesenchymal stem cells (mscs) from adipose tissue, examine the expression of surface markers on these cells, and determine their ability to differentiate into osteoblasts in normal medium. objectives the objectives of this study were to separate and culture mesenchymal stem cells (mscs) from adipose tissue, examine the expression of surface markers on these cells, and determine their ability to differentiate into osteoblasts in normal medium.

Upgrade to premium to download articles

Sign up to access the full text

Already have an account?login

similar resources

Isolation of Adipose Tissue Stem Cells with Organ Culture Method

Background  Isolation of adipose tissue mesenchymal stem cells (MSCs) with current enzymatic methods has some limitations. For example, it is costly and time-consuming and results in a heterogeneous cell population that making compromise proliferation and differentiation of MSCs. Also, it is accompanied with the increased risk of culture contaminations because of several handling steps.In this...

full text

Isolation, culture and induced differentiation of rabbit mesenchymal stem cells into osteoblasts

Mesenchymal stem cells (MSCs) may be easily isolated from the bone marrow, and possess multi-lineage differentiation potential and various therapeutic applications. The differentiation of MSCs into osteoblasts is a complex process that is regulated by multiple internal and external factors. In the present study, the differentiation of MSCs isolated from rabbit bone marrow into osteoblasts using...

full text

Isolation, Characterization and Differentiation of Rat Adipose Tissue Derived Mesenchymal Stem Cells

Introduction:   Mesenchymal stem cells have the potential of self-renewal and differentiation into different cell types, including blood cells, heart, nerves and cartilage, and have unlimited power for division. These cells can be obtained from cord, before implantation from fertilized cells and also from various tissues of adults although the differentiation power and the ability to reproduce ...

full text

Will mesenchymal stem cells differentiate into osteoblasts on allograft?

Tissue engineering approaches for bone blocks previously have used synthetic scaffolds. Bone graft (allograft) is used to fill bone defects, but standard processing can lessen this scaffold's osteoinductive potential. We wanted to test if allografts could be used to produce a viable bone block using mesenchymal stem cells. We hypothesized that mesenchymal stem cells differentiate into osteoblas...

full text

Differentiation of Mesenchymal Stem Cells Derived From Human Adipose Tissue into Cholinergic-like Cells: In Vitro Study

Introduction: Cholinergic-associated diseases currently constitute a significant cause of neurological and neurodegenerative disabilities.  As the drugs are not efficient in improving the suffered tissues, stem cell treatment is considered an effective strategy for substituting the lost cells. Methods: In the current study, we set out to investigate the differentiation properties of human adip...

full text

isolation of adipose tissue stem cells with organ culture method

background  isolation of adipose tissue mesenchymal stem cells (mscs) with current enzymatic methods has some limitations. for example, it is costly and time-consuming and results in a heterogeneous cell population that making compromise proliferation and differentiation of mscs. also, it is accompanied with the increased risk of culture contaminations because of several handling steps.in this ...

full text

My Resources

Save resource for easier access later


Journal title:
avicenna journal of dental research

جلد ۷، شماره ۲، صفحات ۰-۰

Hosted on Doprax cloud platform doprax.com

copyright © 2015-2023